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• For intra-vaginal progesterone releasing devices to be effective in synchronizing estrus in, they must increase progesterone in blood. Our study showed that reused CIDRs do not increase serum progesterone and therefore are ineffective in synchronizing estrus in cattle.

• Reused CIDRs have a higher loss rate and are more strongly associated with vaginitis than new CIDRs.

• There was no statistical association between days open, days in milk, lactation number, milk weight and mature equivalent milk production and serum progesterone levels.

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